| Division | Experiment subject | Experimental results |
|---|---|---|
| In vitro | Cell proliferation evaluation | Highest result in hot water extract of Humulus japonicus(Humulus japonica Sidb. et Zucc.) |
| ALP(alkaline phosphatase): Growth factors that are particularly high during the period of growth at the time of osteogenesis promoting action | High results in A(Humulus japonicus[Humulus japonica Sidb. et Zucc.] hot water extract) and A+B(Humulus japonicus[Humulus japonica Sidb. et Zucc.] hot water extract 8: watermelon/garlic 2) | |
| Osteocalcin: Growth factors that increase bone density and act on osteogenesis | High results in A(Humulus japonicus[Humulus japonica Sidb. et Zucc.] hot water extract)and A+B(Humulus japonicus[Humulus japonica Sidb. et Zucc.] hot water extract 8: watermelon/garlic 2) | |
| IGF-1(Insulin-like growth factor 1): Growth promoter that is similar in structure to insulin | High results in A(Humulus japonicus[Humulus japonica Sidb. et Zucc.] hot water extract) and A+B(Humulus japonicus[Humulus japonica Sidb. et Zucc.] hot water extract 8: watermelon/garlic 2) | |
| In vivo | Confirmation of changes in body length and the growth factors of normal rats by oral administration of Humulus japonicu(Humulus japonica Sidb. et Zucc.) and watermelon/garlic. | Compared to ND, increased growth factors affecting rat body length and growth in the group administered growth hormone and A(Humulus japonicus[Humulus japonica Sidb. et Zucc.] hot water extract) and A+B(Humulus japonicus[Humulus japonica Sidb. et Zucc.] hot water extract 8: watermelon/garlic 2). Especially, the meaningful growth appeared with A(Humulus japonicus[Humulus japonica Sidb. et Zucc.] hot water extract) and A+B(Humulus japonicus[Humulus japonica Sidb. et Zucc.] hot water extract 8: watermelon/garlic 2). |
| Confirmation of changes in body length and the growth factors of hypophysectomized rats by oral administration of Humulus japonicu(Humulus japonica Sidb. et Zucc.) and watermelon/garlic. | In the group administered A(Humulus japonicus[Humulus japonica Sidb. et Zucc.] hot water extract) and A+B(Humulus japonicus[Humulus japonica Sidb. et Zucc.] hot water extract 8: watermelon/garlic 2) to hypophysectomized rats, Growth factors affecting body length and growth in rats did not significantly grow. However, significant growth occurs in the growth hormone administration group. It was confirmed that A(Humulus japonicus[Humulus japonica Sidb. et Zucc.] hot water extract) and A+B(Humulus japonicus[Humulus japonica Sidb. et Zucc.] hot water extract 8: watermelon/garlic 2) had no significant correlation with the role of growth hormone. Confirmed safety as a food material. |
Cell proliferation evaluation
Changes in ALP gene expression
Changes in Osteocalcin gene expression
Changes in IGF-1 concentration
▶ Measurement result of cell proliferation evaluation, ALP gene expression, Osteocalcin Gene Expression, IGF-1 concentration shows high result in Humulus japonicus extract.
Rat bone length change (Femur)
Rat bone length change (Tibia)
Rat bone tissue H&E stain results
Micro-CT image of Rat tibia
▶ Compared to ND (normal control),The body length, growth plate height, and growth factors IGF-1 and IGFBP-3 were increased in Rat administered with growth hormone and Humulus japonicus(Humulus japonica Sidb. et Zucc.), and Humulus japonicus(Humulus japonica Sidb. et Zucc.) mixture.
Rat bone length change (Femur)
Rat Bone length change (Tibia)
Rat bone tissue H&E stain results
Micro-CT image of Rat tibia
▶ In the group administered Humulus japonicus(Humulus japonica Sidb. et Zucc.), Humulus japonicus(Humulus japonica Sidb. et Zucc.) mixture to hypophysectomized rats,
Longitudinal Bone Growth, growth plate height, Growth factors, IGF-1, and IGFBP-3 in rats did not significantly grow.
However, significant growth in the growth hormone administration group.
Confirmed that Humulus japonicus(Humulus japonica Sidb. et Zucc.), Humulus japonicus(Humulus japonica Sidb. et Zucc.) mixture had no significant correlation with the role of growth hormone.
▶ Confirmed safety as a food material.